AFM DATA ANALYSIS

Individual AFM force spectroscopy measurements were analysed using PUNIAS (punias.fr) software in protein unfolding mode. To analyse the curve, the baseline was specified and any wrongly identified adhesion events marked or unmarked accordingly. PUNIAS outputs a summary data file which contains the measurements for every individual force spectrum.  

Excel file AFM data_compilation is comprised of values measured for force (pN), length (nm), and total work (pN.nm) with PUNIAS analysis for 3 individual Fn-functionalised tips. 
One sheet of data per individual sample (i.e. AT-PCL 25, AT-PCL 25 doped), while the following sample abbreviations were used:

25% AT-PCL = 25
25% AT-PCL doped = 25D
50% AT-PCL = 50
50% AT-PCL doped = 50D
75% AT-PCL = 75
75% AT-PCL doped = 75D

 